Intracellular distribution of low molecular weight RNA species in HeLa cells.

Intracellular distribution of low molecular weight RNA species in HeLa cells

Intracellular distributions of the low molecular weight RNA species of HeLa cells were determined by a nonaqueous method of cell fractionation, in which lyophilized cells were homogenized and centrifuged in anhydrous glycerol. The nonaqueous method was used to avoid artifactual extraction of weakly bound nuclear RNA during cell fractionation. We found that the mature small RNA species K, A, C, ...

Intracellular Distribution of Low Molecular Weight Rna in Chironomus Tentans

Low molecular weight RNA species are described in isolated nuclear components and cytoplasm of salivary gland cells of Chironomus tentans. In addition to 4S and 5S RNA and RNA in the 4-5S range previously described, at least three other components in the range below 16S are present. RNA, the molecular weight of which was estimated to 2.3 x 10(5) and designed 10S RNA, can be observed only in nuc...

High molecular weight nonribosomal-type nuclear RNA and cytoplasmic messenger RNA in HeLa cells.

As reported elsewhere,' the study of RNA synthesis in vitro in immature duck erythrocytes has revealed the occurrence in the nucleus of fast turning-over RNA of high molecular weight (from 2 X 106 to 107, or possibly more) and with base composition different from that of ribosomal RNA, and characterized by a high U and relatively low GC content. No relationship of precursor-to-product typeor, a...

Modeling Spatial Distribution of Intracellular Organelles by Molecular Motors

Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecular weight protamine

BACKGROUND Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention and treatment of various diseases. However, the instability of CPPs and mRNAs has limited their application. Bacteriophage PP7 virus-like particles (VLPs) may protect peptides an...